Molecular epidemiology of the Staphylococcus aureus by Rep-PCR method in Sanandaj hospitals

نویسنده

  • Rashid Ramazanzadeh
چکیده

Staphylococcus aureusis a major pathogen within hospital and community. The aim of this study was genetic relationship determination in isolates bacteria and dynamic transmission in hospital. Eighty eight S. aureusstrains isolated from different clinical samples and were characterized by Repetitive extragenic palindromic (Rep–PCR) technique. The received results and the similarity between the strains were determined on the basis of the Jaccard similarity coefficient in the SAHN program of the NTSYS-pc software. The Rep–PCR profile allowed the typing of the 88 isolates into 7 main clusters. In conclusion, our results showed more diversity in S.aureus isolates that indicates the low rate of hospital infection in Sanandaj hospitals and the results of the share pattern of especially among ICU, Pediateric and Internal wardsindicate the exist of nosocomial infections in these wards, According to the study that we carried out the greatest resistance was observed to erythromycinAnd for all drives vancomycin-resistant, MIC of vancomycin using E-test strips were placed by all who are sensitive, so that the Disc Diffusions are not reliable at all. [Rashid Ramazanzadeh, Hanar Narenji.Molecular epidemiology of the Staphylococcus aureus by Rep-PCR method in Sanandaj hospitals. Life Sci J 2013;10(12s):117-121]. (ISSN:1097-8135). http://www.lifesciencesite.com. 20

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تاریخ انتشار 2013